Yuan-Tzu Ou-Yang*
Department of Life Science, National Taiwan Normal University, Taiwan.
*Corresponding author: Yuan-Tzu Ou-Yang, oyyz.mail@gmail.com
Keywords: Chromatography, Photosynthetic pigments, Color mixture theory
The conventional chemical colorimetric method compares the absorbance or transmittance of the solution to be analyzed. A series of standard solutions of different concentrations have been prepared until the absorbance or transmittance of the unknown concentration solution is the same as that of a standard solution in the concentration of this unknown solution. Primary photosynthetic pigments in leaves contain chlorophyll a, chlorophyll b, carotenoids, and lutein. The conventional determination methods include rotten leaves, solution extraction, and chromatographic analysis. The chromatographic analysis employed affinity for solvents to photosynthetic pigments in leaves to produce the color spectrum solution in test strips or tubes. The full pre-calibrated spectrum of solution colors corresponding to the specific pigments would deduce the content of photosynthetic pigments based on the formula proposed by Aron et al. Obviously; this method is time-consuming, complicated, and destructive. By grabbing from the color leaf images, this study intends to calculate the content of each photosynthetic pigment which could predict the color leaf images based on color mixture theory and AI prediction model by the pre-calibrated color coordination in photosynthetic pigments. It is hoped that the content of each photosynthetic pigment in leaves can be determined by a simple, fast, accurate, and non-destructive method.
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